Dataset Information

One-Step Fabrication of Three-Dimensional Fibrous Collagen-Based Macrostructure with High Water Uptake Capability by Coaxial Electrospinning.

ABSTRACT: One step fabrication of the three dimension (3D) fibrous structure of Collagen-g-poly(MMA-co-EA)/Nylon6 was investigated by controlling the experimental conditions during coaxial electrospinning. This 3D fibrous structure is the result of interactions of two polymeric systems with a varied capability to be electrostatically polarized under the influence of the external electric field; the solution with the higher conductivity into the inner spinneret and the solution with the lesser conductivity into the outer capillary of the coaxial needle. This set-up was to obtain bimodal fiber fabrication in micro and nanoscale developing a spatial structure; the branches growing off a trunk. The resultant 3D collagen-based fibrous structure has two distinguished configurations: microfibers of 6.9 ± 2.2 µm diameter gap-filled with nanofibers of 216 ± 49 nm diameter. The 3D fibrous structure can be accumulated at an approximate height of 4 cm within 20 min. The mechanism of the 3D fibrous structure and the effect of experimental conditions, the associated hydration degree, water uptake and degradation rate were also investigated. This highly stable 3D fibrous structure has great potential end-uses benefitting from its large surface area and high water uptake which is caused by the high polarity and spatial orientation of collagen-based macrostructure.

SUBMITTER: Bazrafshan Z

PROVIDER: S-EPMC6215112 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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One-Step Fabrication of Three-Dimensional Fibrous Collagen-Based Macrostructure with High Water Uptake Capability by Coaxial Electrospinning.

Bazrafshan Zahra Z Stylios George K GK

Nanomaterials (Basel, Switzerland) 20181008 10

One step fabrication of the three dimension (3D) fibrous structure of Collagen-g-poly(MMA-co-EA)/Nylon6 was investigated by controlling the experimental conditions during coaxial electrospinning. This 3D fibrous structure is the result of interactions of two polymeric systems with a varied capability to be electrostatically polarized under the influence of the external electric field; the solution with the higher conductivity into the inner spinneret and the solution with the lesser conductivity ...[more]

PMID: 30297671

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Project description:While tissue form and function is highly dependent upon tissue-specific collagen composition and organization, little is known of the mechanisms controlling the bundling of collagen fibrils into fibers and larger structural designs that lead to the formation of bones, tendons and other tissues. Using the cornea as a model system, our previous 3 dimensional mapping of collagen fiber organization has demonstrated that macrostructural organization of collagen fibers involving interweaving, branching and anastomosing plays a critical role in controlling mechanical stiffness, corneal shape and refractive power. In this work, the cellular and mechanical mechanisms regulating critical events in the assembly of collagen macrostructure are analysed in the developing chicken cornea. We elucidated the temporal events leading to adult corneal structure and determined the effects of intraocular pressure (IOP) on the organization of the collagen macrostructure. Our findings indicate that the complex adult collagen organization begins to appear on embryonic day 10 (E10) after deposition of the primary stroma and full invasion of keratocytes. Importantly, organizational changes in keratocytes appearing at E9 preceded and predicted later changes in collagen organization. Corneal collagen organization remained unaffected when the development of IOP was blocked at E4. These findings support a primary role for keratocytes in controlling stromal organization, mechanical stiffness and corneal shape that are not regulated by the IOP. Our findings also suggest that the avian cornea represents an excellent experimental model for elucidating key regulatory steps and mechanisms controlling the collagen fiber organization that is critical to determining tissue form and function. STATEMENT OF SIGNIFICANCE:This work by using an ex ovo model system, begins to investigate the potential mechanisms controlling collagen fibril macrostructure. In particular, this work highlights a convergent role for the corneal keratocytes in organizing the complex collagen macrostructure, necessary to support high visual acuity. Our data supports that the intraocular pressure does not influence collagen fibril macrostructure and suggest that the avian cornea represents an excellent experimental model for elucidating key regulatory steps and mechanisms controlling the collagen fiber organization that is critical to determining tissue form and function. Clearly understanding the cellular and molecular mechanisms that underlie collagen fibril macrostructure will be highly beneficial for future tissue engineering and regenerative medicine applications.

Dataset Information

One-Step Fabrication of Three-Dimensional Fibrous Collagen-Based Macrostructure with High Water Uptake Capability by Coaxial Electrospinning.

Publications

One-Step Fabrication of Three-Dimensional Fibrous Collagen-Based Macrostructure with High Water Uptake Capability by Coaxial Electrospinning.

Similar Datasets

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