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High-throughput analysis and protein engineering using microcapillary arrays.


ABSTRACT: We describe a multipurpose technology platform, termed ?SCALE (microcapillary single-cell analysis and laser extraction), that enables massively parallel, quantitative biochemical and biophysical measurements on millions of protein variants expressed from yeast or bacteria. ?SCALE spatially segregates single cells within a microcapillary array, enabling repeated imaging, cell growth and protein expression. We performed high-throughput analysis of cells and their protein products using a range of fluorescent assays, including binding-affinity measurements and dynamic enzymatic assays. A precise laser-based extraction method allows rapid recovery of live clones and their genetic material from microcapillaries for further study. With ?SCALE, we discovered a new antibody against a clinical cancer target, evolved a fluorescent protein biosensor and engineered an enzyme to reduce its sensitivity to its inhibitor. These protein analysis and engineering applications each have unique assay requirements and different host organisms, highlighting the flexibility and technical capabilities of the ?SCALE platform.

SUBMITTER: Chen B 

PROVIDER: S-EPMC6215714 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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High-throughput analysis and protein engineering using microcapillary arrays.

Chen Bob B   Lim Sungwon S   Kannan Arvind A   Alford Spencer C SC   Sunden Fanny F   Herschlag Daniel D   Dimov Ivan K IK   Baer Thomas M TM   Cochran Jennifer R JR  

Nature chemical biology 20151207 2


We describe a multipurpose technology platform, termed μSCALE (microcapillary single-cell analysis and laser extraction), that enables massively parallel, quantitative biochemical and biophysical measurements on millions of protein variants expressed from yeast or bacteria. μSCALE spatially segregates single cells within a microcapillary array, enabling repeated imaging, cell growth and protein expression. We performed high-throughput analysis of cells and their protein products using a range of  ...[more]

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