Ontology highlight
ABSTRACT:
SUBMITTER: Chen B
PROVIDER: S-EPMC6215714 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Chen Bob B Lim Sungwon S Kannan Arvind A Alford Spencer C SC Sunden Fanny F Herschlag Daniel D Dimov Ivan K IK Baer Thomas M TM Cochran Jennifer R JR
Nature chemical biology 20151207 2
We describe a multipurpose technology platform, termed μSCALE (microcapillary single-cell analysis and laser extraction), that enables massively parallel, quantitative biochemical and biophysical measurements on millions of protein variants expressed from yeast or bacteria. μSCALE spatially segregates single cells within a microcapillary array, enabling repeated imaging, cell growth and protein expression. We performed high-throughput analysis of cells and their protein products using a range of ...[more]