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Nanovolume optimization of protein crystal growth using the microcapillary protein crystallization system.

ABSTRACT: The Microcapillary Protein Crystallization System (MPCS) is a microfluidic, plug-based crystallization technology that generates X-ray diffraction-ready protein crystals in nanolitre volumes. In this study, 28 out of 29 (93%) proteins crystallized by traditional vapor diffusion experiments were successfully crystallized by chemical gradient optimization experiments using the MPCS technology. In total, 90 out of 120 (75%) protein/precipitant combinations leading to initial crystal hits from vapor diffusion experiments were successfully crystallized using MPCS technology. Many of the resulting crystals produced high-quality X-ray diffraction data, and six novel protein structures that were derived from crystals harvested from MPCS CrystalCards are reported.

SUBMITTER: Gerdts CJ

PROVIDER: S-EPMC3263815 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

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Nanovolume optimization of protein crystal growth using the microcapillary protein crystallization system.

Gerdts Cory J CJ Stahl Glenn L GL Napuli Alberto A Staker Bart B Abendroth Jan J Edwards Thomas E TE Myler Peter P Van Voorhis Wesley W Nollert Peter P Stewart Lance J LJ

Journal of applied crystallography 20100819 Pt 5

The Microcapillary Protein Crystallization System (MPCS) is a microfluidic, plug-based crystallization technology that generates X-ray diffraction-ready protein crystals in nanolitre volumes. In this study, 28 out of 29 (93%) proteins crystallized by traditional vapor diffusion experiments were successfully crystallized by chemical gradient optimization experiments using the MPCS technology. In total, 90 out of 120 (75%) protein/precipitant combinations leading to initial crystal hits from vapor ...[more]

PMID: 22477778

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