Ontology highlight
ABSTRACT:
SUBMITTER: Marie R
PROVIDER: S-EPMC6217438 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
Marie Rodolphe R Pedersen Jonas N JN Bærlocher Loic L Koprowska Kamila K Pødenphant Marie M Sabatel Céline C Zalkovskij Maksim M Mironov Andrej A Bilenberg Brian B Ashley Neil N Flyvbjerg Henrik H Bodmer Walter F WF Kristensen Anders A Mir Kalim U KU
Proceedings of the National Academy of Sciences of the United States of America 20181015 44
To elucidate cellular diversity and clonal evolution in tissues and tumors, one must resolve genomic heterogeneity in single cells. To this end, we have developed low-cost, mass-producible micro-/nanofluidic chips for DNA extraction from individual cells. These chips have modules that collect genomic DNA for sequencing or map genomic structure directly, on-chip, with denaturation-renaturation (D-R) optical mapping [Marie R, et al. (2013) <i>Proc Natl Acad Sci USA</i> 110:4893-4898]. Processing o ...[more]