Profiling and bioinformatics analyses of differential circular RNA expression in prostate cancer cells.
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ABSTRACT: Aim
There is little knowledge about the expression profile and function of circular RNAs (circRNAs) in prostate cancer (PCa).Methods
The expression profiles of circRNAs in RWPE-1, 22RV1 and PC3 cells were explored via high-throughput circRNAs sequencing and validated by real-time qPCR. The roles of differentially expressed circRNAs were evaluated by bioinformatics analyses.Results
Altogether 9545 circRNAs were identified and hundreds of differentially expressed circRNAs were recognized. CircRNA-miRNA networks analysis showed that many circRNAs, including circSLC7A6, circGUCY1A2 and circZFP57 could cross-talk with tumor-related miRNAs such as miR-21, miR-143 and miR-200 family.Conclusion
The results of our bioinformatics analyses suggested that circRNAs should play critical roles in the development and progression of PCa.
SUBMITTER: Zhang C
PROVIDER: S-EPMC6222276 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
Publications
Profiling and bioinformatics analyses of differential circular RNA expression in prostate cancer cells.
Zhang Chunlei C Xiong Jun J Yang Qi Q Wang Ye Y Shi Haoqing H Tian Qinqin Q Huang Hai H Kong Depei D Lv Jianmin J Liu Dan D Gao Xu X Zi Xiaoyuan X Sun Yinghao Y
Future science OA 20181003 9
<h4>Aim</h4>There is little knowledge about the expression profile and function of circular RNAs (circRNAs) in prostate cancer (PCa).<h4>Methods</h4>The expression profiles of circRNAs in RWPE-1, 22RV1 and PC3 cells were explored via high-throughput circRNAs sequencing and validated by real-time qPCR. The roles of differentially expressed circRNAs were evaluated by bioinformatics analyses.<h4>Results</h4>Altogether 9545 circRNAs were identified and hundreds of differentially expressed circRNAs w ...[more]
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