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Proteins on Supported Lipid Bilayers Diffusing around Proteins Fixed on Acrylate Anchors.

ABSTRACT: Mobility of proteins and lipids plays a major role in physiological processes. Platforms which were developed to study protein interaction between immobilized and mobile proteins suffer from shortcomings such as fluorescence quenching or complicated fabrication methods. Here we report a versatile platform comprising immobilized histidine-tagged proteins and biotinylated proteins in a mobile phase. Importantly, multiphoton photolithography was used for easy and fast fabrication of the platform and allows, in principle, extension of its application to three dimensions. The platform, which is made up of functionalized polymer structures embedded in a mobile lipid bilayer, shows low background fluorescence and allows for mobility of arbitrary proteins.

SUBMITTER: Buchegger B

PROVIDER: S-EPMC6222595 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Proteins on Supported Lipid Bilayers Diffusing around Proteins Fixed on Acrylate Anchors.

Buchegger Bianca B Kreutzer Johannes J Axmann Markus M Mayr Sandra S Wollhofen Richard R Plochberger Birgit B Jacak Jaroslaw J Klar Thomas A TA

Analytical chemistry 20181025 21

Mobility of proteins and lipids plays a major role in physiological processes. Platforms which were developed to study protein interaction between immobilized and mobile proteins suffer from shortcomings such as fluorescence quenching or complicated fabrication methods. Here we report a versatile platform comprising immobilized histidine-tagged proteins and biotinylated proteins in a mobile phase. Importantly, multiphoton photolithography was used for easy and fast fabrication of the platform an ...[more]

PMID: 30350628

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