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High-resolution ultramicroscopy of the developing and adult nervous system in optically cleared Drosophila melanogaster.


ABSTRACT: The fruit fly, Drosophila melanogaster, is an important experimental model to address central questions in neuroscience at an organismic level. However, imaging of neural circuits in intact fruit flies is limited due to structural properties of the cuticle. Here we present a novel approach combining tissue clearing, ultramicroscopy, and data analysis that enables the visualisation of neuronal networks with single-cell resolution from the larval stage up to the adult Drosophila. FlyClear, the signal preserving clearing technique we developed, stabilises tissue integrity and fluorescence signal intensity for over a month and efficiently removes the overall pigmentation. An aspheric ultramicroscope set-up utilising an improved light-sheet generator allows us to visualise long-range connections of peripheral sensory and central neurons in the visual and olfactory system. High-resolution 3D reconstructions with isotropic resolution from entire GFP-expressing flies are obtained by applying image fusion from orthogonal directions. This methodological integration of novel chemical, optical, and computational techniques allows a major advance in the analysis of global neural circuit organisation.

SUBMITTER: Pende M 

PROVIDER: S-EPMC6226481 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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A 3-year Mediterranean-style dietary intervention may modulate the association between adiponectin gene variants and body weight change.

Razquin C C   Martínez J A JA   Martínez-González M A MA   Salas-Salvadó J J   Estruch R R   Marti A A  

European journal of nutrition 20091225 5


<h4>Purpose</h4>Adiponectin gene variations have been associated with obesity. There are few interventional studies analyzing this association. The aim of this study was to analyze the effects of a nutritional intervention with Mediterranean-style diet and three (-4034A/C, +45T/G, and +276 G/T) adiponectin gene variants on 3-year body weight changes in high cardiovascular risk patients.<h4>Subjects and methods</h4>A total of 737 participants, aged 55-80 at high cardiovascular risk were assigned  ...[more]

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