Luminescence lifetime encoding in time-domain flow cytometry.
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ABSTRACT:
SUBMITTER: Kage D
PROVIDER: S-EPMC6233182 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Publications
Luminescence lifetime encoding in time-domain flow cytometry.
Kage Daniel D Hoffmann Katrin K Wittkamp Marc M Ameskamp Jens J Göhde Wolfgang W Resch-Genger Ute U
Scientific reports 20181113 1
Time-resolved flow cytometry represents an alternative to commonly applied spectral or intensity multiplexing in bioanalytics. At present, the vast majority of the reports on this topic focuses on phase-domain techniques and specific applications. In this report, we present a flow cytometry platform with time-resolved detection based on a compact setup and straightforward time-domain measurements utilizing lifetime-encoded beads with lifetimes in the nanosecond range. We provide general assessme ...[more]
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