Ontology highlight
ABSTRACT:
SUBMITTER: Jiang Y
PROVIDER: S-EPMC6235398 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Jiang Yiyang Y Yu Hailong H Li Fudong F Cheng Lin L Zhu Lingru L Shi Yunyu Y Gong Qingguo Q
PLoS pathogens 20181102 11
Methyltransferase RlmCD was previously shown to be responsible for the introduction of C5 methylation at both U747 and U1939 of the 23S ribosomal RNA in Streptococcus pneumoniae. Intriguingly, its structural homologue, RumA, can only catalyze the methylation of U1939, while RlmC is the dedicated enzyme for m5U747 in Escherichia coli. In this study, we describe the structure of RlmCD in complex with its cofactor and the RNA substrate containing U747 at 2.00 Å or U1939 at 3.10 Å. We demonstrate th ...[more]