Ontology highlight
ABSTRACT:
SUBMITTER: Hegedus E
PROVIDER: S-EPMC6237785 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Hegedüs Éva É Kókai Endre E Nánási Péter P Imre László L Halász László L Jossé Rozenn R Antunovics Zsuzsa Z Webb Martin R MR El Hage Aziz A Pommier Yves Y Székvölgyi Lóránt L Dombrádi Viktor V Szabó Gábor G
Nucleic acids research 20181101 20
Molecular combing and gel electrophoretic studies revealed endogenous nicks with free 3'OH ends at ∼100 kb intervals in the genomic DNA (gDNA) of unperturbed and G1-synchronized Saccharomyces cerevisiae cells. Analysis of the distribution of endogenous nicks by Nick ChIP-chip indicated that these breaks accumulated at active RNA polymerase II (RNAP II) promoters, reminiscent of the promoter-proximal transient DNA breaks of higher eukaryotes. Similar periodicity of endogenous nicks was found with ...[more]