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Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy.

ABSTRACT: Imaging and tracking of near-surface three-dimensional volumetric nanoscale dynamic processes of live cells remains a challenging problem. In this paper, we propose a multi-color live-cell near-surface-volume super-resolution microscopy method that combines total internal reflection fluorescence structured illumination microscopy with multi-angle evanescent light illumination. We demonstrate that our approach of multi-angle interference microscopy is perfectly adapted to studying subcellular dynamics of mitochondria and microtubule architectures during cell migration.

SUBMITTER: Chen Y

PROVIDER: S-EPMC6240104 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy.

Chen Youhua Y Liu Wenjie W Zhang Zhimin Z Zheng Cheng C Huang Yujia Y Cao Ruizhi R Zhu Dazhao D Xu Liang L Zhang Meng M Zhang Yu-Hui YH Fan Jiannan J Jin Luhong L Xu Yingke Y Kuang Cuifang C Liu Xu X

Nature communications 20181116 1

Imaging and tracking of near-surface three-dimensional volumetric nanoscale dynamic processes of live cells remains a challenging problem. In this paper, we propose a multi-color live-cell near-surface-volume super-resolution microscopy method that combines total internal reflection fluorescence structured illumination microscopy with multi-angle evanescent light illumination. We demonstrate that our approach of multi-angle interference microscopy is perfectly adapted to studying subcellular dyn ...[more]

PMID: 30446673

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