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Engineering yeast endosymbionts as a step toward the evolution of mitochondria.


ABSTRACT: It has been hypothesized that mitochondria evolved from a bacterial ancestor that initially became established in an archaeal host cell as an endosymbiont. Here we model this first stage of mitochondrial evolution by engineering endosymbiosis between Escherichia coli and Saccharomyces cerevisiae An ADP/ATP translocase-expressing E. coli provided ATP to a respiration-deficient cox2 yeast mutant and enabled growth of a yeast-E. coli chimera on a nonfermentable carbon source. In a reciprocal fashion, yeast provided thiamin to an endosymbiotic E. coli thiamin auxotroph. Expression of several SNARE-like proteins in E. coli was also required, likely to block lysosomal degradation of intracellular bacteria. This chimeric system was stable for more than 40 doublings, and GFP-expressing E. coli endosymbionts could be observed in the yeast by fluorescence microscopy and X-ray tomography. This readily manipulated system should allow experimental delineation of host-endosymbiont adaptations that occurred during evolution of the current, highly reduced mitochondrial genome.

SUBMITTER: Mehta AP 

PROVIDER: S-EPMC6243291 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Engineering yeast endosymbionts as a step toward the evolution of mitochondria.

Mehta Angad P AP   Supekova Lubica L   Chen Jian-Hua JH   Pestonjamasp Kersi K   Webster Paul P   Ko Yeonjin Y   Henderson Scott C SC   McDermott Gerry G   Supek Frantisek F   Schultz Peter G PG  

Proceedings of the National Academy of Sciences of the United States of America 20181029 46


It has been hypothesized that mitochondria evolved from a bacterial ancestor that initially became established in an archaeal host cell as an endosymbiont. Here we model this first stage of mitochondrial evolution by engineering endosymbiosis between <i>Escherichia coli</i> and <i>Saccharomyces cerevisiae</i> An ADP/ATP translocase-expressing <i>E. coli</i> provided ATP to a respiration-deficient <i>cox2</i> yeast mutant and enabled growth of a yeast-<i>E. coli</i> chimera on a nonfermentable ca  ...[more]

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