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Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism.

ABSTRACT: [FeFe]-Hydrogenases are hydrogen producing metalloenzymes with excellent catalytic capacities, highly relevant in the context of a future hydrogen economy. Here we demonstrate the synthetic activation of a heterologously expressed [FeFe]-hydrogenase in living cells of Synechocystis PCC 6803, a photoautotrophic microbial chassis with high potential for biotechnological energy applications. H2-Evolution assays clearly show that the non-native, semi-synthetic enzyme links to the native metabolism in living cells.

SUBMITTER: Wegelius A

PROVIDER: S-EPMC6243476 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism.

Wegelius Adam A Khanna Namita N Esmieu Charlène C Barone Giovanni Davide GD Pinto Filipe F Tamagnini Paula P Berggren Gustav G Lindblad Peter P

Energy & environmental science 20180925 11

[FeFe]-Hydrogenases are hydrogen producing metalloenzymes with excellent catalytic capacities, highly relevant in the context of a future hydrogen economy. Here we demonstrate the synthetic activation of a heterologously expressed [FeFe]-hydrogenase in living cells of <i>Synechocystis</i> PCC 6803, a photoautotrophic microbial chassis with high potential for biotechnological energy applications. H<sub>2</sub>-Evolution assays clearly show that the non-native, semi-synthetic enzyme links to the n ...[more]

PMID: 30555530

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