Automated sequential chromogenic IHC double staining with two HRP substrates.
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ABSTRACT:
SUBMITTER: Petersen KH
PROVIDER: S-EPMC6245840 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Publications
Automated sequential chromogenic IHC double staining with two HRP substrates.
Petersen Kenneth Heesche KH Lohse Jesper J Ramsgaard Lasse L
PloS one 20181120 11
Automated IHC double staining using diaminobenzidine and HRP Magenta is illustrated utilizing a new acidic block with sulfuric acid to prevent cross-reactivity. Residual cross-reactivity in double staining is determined to arise from chromogenic-bound antibodies and amplification system during the first part of the double staining. ...[more]
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