Ontology highlight
ABSTRACT:
SUBMITTER: Henderson WA
PROVIDER: S-EPMC6253687 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
Henderson Wendy A WA Xiang Lichen L Fourie Nicolaas H NH Abey Sarah K SK Ferguson Eric G EG Diallo Ana F AF Kenea Natnael D ND Kim Chang Hee CH
Analytical methods : advancing methods and applications 20180920 45
Diarrheal diseases claim the lives of 1300 children daily, mostly in the developing world. We have developed a simple lateral flow assay capable of detecting <i>E. coli</i> and EPEC DNA and RNA rapidly (<15 minutes) at the point-of-need, directly from stool without nucleic acid extraction or molecular amplification. The limit of detection of the method is 1 nM using synthetic DNA target substrates spiked into stool. However, due to the endogenous amplification of the 23S rRNA targets, we were ab ...[more]