Ontology highlight
ABSTRACT:
SUBMITTER: Guo C
PROVIDER: S-EPMC6258002 | biostudies-literature | 2017 Feb
REPOSITORIES: biostudies-literature
Guo Changmiao C Hou Guangjin G Lu Xingyu X Polenova Tatyana T
Journal of biomolecular NMR 20170124 2
REDOR-based experiments with simultaneous <sup>1</sup>H-<sup>13</sup>C and <sup>1</sup>H-<sup>15</sup>N dipolar dephasing are explored for investigating intermolecular protein-protein interfaces in complexes formed by a U-<sup>13</sup>C,<sup>15</sup>N-labeled protein and its natural abundance binding partner. The application of a double-REDOR filter (dREDOR) results in a complete dephasing of proton magnetization in the U-<sup>13</sup>C,<sup>15</sup>N-enriched molecule while the proton magnetiza ...[more]