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Stabilization of the p53-DNA Complex by the Nuclear Protein Dmp1?.

ABSTRACT: We recently reported the existence of a physical interaction between the Myb-like transcription factor Dmp1 (Dmtf1) and p53 in which Dmp1 antagonized polyubiquitination of p53 by Mdm2 and promoted its nuclear localization. Dmp1 significantly stabilized p53-DNA complexes on promoters that contained p53-consensus sequences, which were either supershifted or disrupted with antibodies to Dmp1. Lysates from mice injected with doxorubicin showed that Dmp1 bound to p21Cip1, Bbc3, and Thbs1 gene regulatory regions in a p53-dependent fashion. Our data suggest that acceleration of DNA-binding of p53 by Dmp1 is a critical process for Dmp1 to increase the p53 function in Arf-deficient cells.

SUBMITTER: Kendig RD

PROVIDER: S-EPMC6262109 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

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Stabilization of the p53-DNA Complex by the Nuclear Protein Dmp1α.

Kendig Robert D RD Kai Fumitake F Fry Elizabeth A EA Inoue Kazushi K

Cancer investigation 20170413 5

We recently reported the existence of a physical interaction between the Myb-like transcription factor Dmp1 (Dmtf1) and p53 in which Dmp1 antagonized polyubiquitination of p53 by Mdm2 and promoted its nuclear localization. Dmp1 significantly stabilized p53-DNA complexes on promoters that contained p53-consensus sequences, which were either supershifted or disrupted with antibodies to Dmp1. Lysates from mice injected with doxorubicin showed that Dmp1 bound to p21<sup>Cip1</sup>, Bbc3, and Thbs1 g ...[more]

PMID: 28406729

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