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Synthesis of a dual-labeled probe of dimethyl lithospermate B with photochemical and fluorescent properties.

ABSTRACT: Dimethyl lithosermate B (DLB) is a highly potent natural antioxidant and antidiabetic polyphenol with unknown mode of action. To determine its cellular targets, a photochemical and fluorescent dimethyl lithopermate B probe was designed and efficiently synthesized. The dual-labeled chemical probe for biological application was evaluated by UV and fluorescence to determine its electrochemical absorption and emission properties. This probe could be valuable for investigating ligand-protein interactions and subcellular localization.

SUBMITTER: Lim E

PROVIDER: S-EPMC6264775 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature

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Synthesis of a dual-labeled probe of dimethyl lithospermate B with photochemical and fluorescent properties.

Lim Eunyoung E Ricci Jeremy J Jung Mankil M

Molecules (Basel, Switzerland) 20111128 12

Dimethyl lithosermate B (DLB) is a highly potent natural antioxidant and antidiabetic polyphenol with unknown mode of action. To determine its cellular targets, a photochemical and fluorescent dimethyl lithopermate B probe was designed and efficiently synthesized. The dual-labeled chemical probe for biological application was evaluated by UV and fluorescence to determine its electrochemical absorption and emission properties. This probe could be valuable for investigating ligand-protein interact ...[more]

PMID: 22124203

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