Ontology highlight
ABSTRACT:
SUBMITTER: Chen B
PROVIDER: S-EPMC6265289 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Chen Baohui B Zou Wei W Xu Haiyue H Liang Ying Y Huang Bo B
Nature communications 20181129 1
The lack of efficient tools to image non-repetitive genes in living cells has limited our ability to explore the functional impact of the spatiotemporal dynamics of such genes. Here, we addressed this issue by developing a CRISPR-Tag system using one to four highly active sgRNAs to specifically label protein-coding genes with a high signal-to-noise ratio for visualization by wide-field fluorescence microscopy. Our approach involves assembling a CRISPR-Tag within the intron region of a fluorescen ...[more]