Ontology highlight
ABSTRACT:
SUBMITTER: Jeon J
PROVIDER: S-EPMC6265457 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Jeon Jongcheol J McGinty Robert K RK Muir Tom W TW Kim Jung-Ae JA Kim Jaehoon J
Nucleic acids research 20181101 21
H2B ubiquitylation (H2Bub)-dependent H3K4 methylation is mediated by the multisubunit Set1 complex (Set1C) in yeast, but precisely how Set1C subunits contribute to this histone modification remains unclear. Here, using reconstituted Set1Cs and recombinant H2Bub chromatin, we identified Set1C subunits and domains involved in the H2Bub-dependent H3K4 methylation process, showing that the Spp1 PHDL domain, in conjunction with the Set1 n-SET domain, interacts with Swd1/Swd3 and that this interaction ...[more]