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Data on preparation and characterization of an insect odorant receptor based biosensor.


ABSTRACT: Insect Odorant receptors (OrXs) can be used as the recognition element in a biosensor as they demonstrate high levels of sensitivity and selectivity towards volatile organic compounds. Herein, we describe a method to express and purify insect odorant receptors and reconstitute them into artificial lipid bilayers (liposomes). These OrX/liposomes were covalently attached to a gold surface and characterized using quartz crystal microbalance with dissipation monitoring (QCM-D). The interaction of OrX/liposomes immobilized on a gold surface to positive and negative odorants were studied by means of electrochemical impedance spectroscopy (EIS) and QCM-D. The data presented in this article are related to the research article titled "An ultrasensitive electrochemical impedance-based biosensor using insect odorant receptors to detect odorants" [1].

SUBMITTER: Khadka R 

PROVIDER: S-EPMC6265521 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Data on preparation and characterization of an insect odorant receptor based biosensor.

Khadka Roshan R   Aydemir Nihan N   Carraher Colm C   Hamiaux Cyril C   Colbert Damon D   Cheema Jamal J   Malmström Jenny J   Kralicek Andrew A   Travas-Sejdic Jadranka J  

Data in brief 20181107


Insect Odorant receptors (OrXs) can be used as the recognition element in a biosensor as they demonstrate high levels of sensitivity and selectivity towards volatile organic compounds. Herein, we describe a method to express and purify insect odorant receptors and reconstitute them into artificial lipid bilayers (liposomes). These OrX/liposomes were covalently attached to a gold surface and characterized using quartz crystal microbalance with dissipation monitoring (QCM-D). The interaction of Or  ...[more]

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