Ontology highlight
ABSTRACT:
SUBMITTER: Li Y
PROVIDER: S-EPMC6269370 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
Li Ya Y Zhang Weifeng W Zhao Junli J Li Sai S Shan Linlin L Zhu Jiuling J Li Yan Y Zhu He H Mao Qinwen Q Xia Haibin H
Cytotechnology 20180901 6
The clustered regulatory interspersed short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) system has been widely used for gene knock-out. Lentiviral vectors have been commonly used as a delivery method for this system, however, prolonged Cas9/sgRNA expression due to lentiviral integration can lead to accumulating off-target mutations. To solve this issue in engineering a gene knock-out cell line, this study established a novel system, which was composed of two lentiviral vectors. O ...[more]