Ontology highlight
ABSTRACT:
SUBMITTER: Chen X
PROVIDER: S-EPMC6071600 | biostudies-other | 2018 Jul
REPOSITORIES: biostudies-other
Chen Xiangyang X Liao Shimiao S Huang Xinya X Xu Ting T Feng Xuezhu X Guang Shouhong S
G3 (Bethesda, Md.) 20180731 8
Rearranged chromosomes have been applied to construct genetic balancers to manipulate essential genes in <i>C. elegans</i> Although much effort has been put into constructing balancer chromosomes, approximately 6% (map units) of the <i>C. elegans</i> genome has not been covered, and this area lies mostly in pairing centers (PCs). Here, we developed a method for conditional chromosomal engineering through combinatorial use of the CRISPR/Cas9 and Cre/<i>LoxP</i> technologies. Functional DNA fragme ...[more]