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Cyclic peptide-capped gold nanoparticles for enhanced siRNA delivery.

ABSTRACT: Previously, we have reported the synthesis of a homochiral l-cyclic peptide [WR]5 and its use for delivery of anti-HIV drugs and biomolecules. A physical mixture of HAuCl4 and the peptide generated peptide-capped gold nanoparticles. Here, [WR]5 and [WR]5-AuNPs were tested for their efficiency to deliver a small interfering RNA molecule (siRNA) in human cervix adenocarcinoma (HeLa) cells. Flow cytometry investigation revealed that the intracellular uptake of a fluorescence-labeled non-targeting siRNA (200 nM) was enhanced in the presence of [WR]5 and [WR]5-AuNPs by 2- and 3.8-fold when compared with that of siRNA alone after 24 h incubation. Comparative toxicity results showed that [WR]5 and [WR]5-AuNPs were less toxic in cells compared to other available carrier systems, such as Lipofectamine.

SUBMITTER: Shirazi AN

PROVIDER: S-EPMC6271229 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature

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Cyclic peptide-capped gold nanoparticles for enhanced siRNA delivery.

Shirazi Amir Nasrolahi AN Paquin Karissa L KL Howlett Niall G NG Mandal Dindyal D Parang Keykavous K

Molecules (Basel, Switzerland) 20140828 9

Previously, we have reported the synthesis of a homochiral l-cyclic peptide [WR]5 and its use for delivery of anti-HIV drugs and biomolecules. A physical mixture of HAuCl4 and the peptide generated peptide-capped gold nanoparticles. Here, [WR]5 and [WR]5-AuNPs were tested for their efficiency to deliver a small interfering RNA molecule (siRNA) in human cervix adenocarcinoma (HeLa) cells. Flow cytometry investigation revealed that the intracellular uptake of a fluorescence-labeled non-targeting s ...[more]

PMID: 25170952

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