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A pair of new antioxidant phenolic acid stereoisomers isolated from danshen injection (lyophilized powder).

ABSTRACT: A pair of new phenolic acid stereoisomers, (R)-norsalvianolic acid L (1) and (S)-norsalvianolic acid L (2), was isolated from the Danshen Injection (lyophilized powder). The structural elucidation and stereochemistry determination were achieved by spectroscopic and chemical methods including 1D, 2D NMR (1H-1H COSY, HSQC and HMBC) and circular dichroism experiments. Their antioxidant activities were assessed by the DPPH· and ABTS·+ scavenging methods in vitro with microplate assay. The IC50 values of 1 were 6.9 and 9.7 ?M respectively, which was close to the control salvianolic acid B (7.8 and 7.1 ?M respectively), while the IC50 values of isomer 2 were 27.1 and 25.3 ?M, respectively.

SUBMITTER: Liu J

PROVIDER: S-EPMC6271289 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature

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A pair of new antioxidant phenolic acid stereoisomers isolated from danshen injection (lyophilized powder).

Liu Jing J Zhao Jianfeng J Dai Zhong Z Lin Ruichao R Wang Gangli G Ma Shuangcheng S

Molecules (Basel, Switzerland) 20140204 2

A pair of new phenolic acid stereoisomers, (R)-norsalvianolic acid L (1) and (S)-norsalvianolic acid L (2), was isolated from the Danshen Injection (lyophilized powder). The structural elucidation and stereochemistry determination were achieved by spectroscopic and chemical methods including 1D, 2D NMR (1H-1H COSY, HSQC and HMBC) and circular dichroism experiments. Their antioxidant activities were assessed by the DPPH· and ABTS·+ scavenging methods in vitro with microplate assay. The IC50 value ...[more]

PMID: 24500008

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