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Effect of Culture Conditions on Metabolite Production of Xylaria sp.

ABSTRACT: Seeking a strategy for triggering the cryptic natural product biosynthesis to yield novel compounds in the plant-associated fungus Xylaria sp., the effect of culture conditions on metabolite production was investigated. A shift in the production of five known cytochalasin-type analogues 1-5 to six new ?-pyrone derivatives, xylapyrones A-F (compounds 6-11), from a solid to a liquid medium was observed. These compounds were identified by analysis of 1D and 2D NMR and HRMS data. Compounds 1-3 showed moderate cytotoxicity against HepG2 and Caski cancer cell lines with IC50 values ranging from 25 to 63 ?M and compounds 4-11 were found to be inactive, with IC50 values>100 ?M.

SUBMITTER: Zhang H

PROVIDER: S-EPMC6272309 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

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Effect of Culture Conditions on Metabolite Production of Xylaria sp.

Zhang Hongqi H Deng Zhangshuang Z Guo Zhiyong Z Peng Yan Y Huang Nianyu N He Haibo H Tu Xuan X Zou Kun K

Molecules (Basel, Switzerland) 20150430 5

Seeking a strategy for triggering the cryptic natural product biosynthesis to yield novel compounds in the plant-associated fungus Xylaria sp., the effect of culture conditions on metabolite production was investigated. A shift in the production of five known cytochalasin-type analogues 1-5 to six new α-pyrone derivatives, xylapyrones A-F (compounds 6-11), from a solid to a liquid medium was observed. These compounds were identified by analysis of 1D and 2D NMR and HRMS data. Compounds 1-3 showe ...[more]

PMID: 25942374

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