Browse
Submit Data
Databases
API
Help

Dataset Information

11 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A Peptoid-Based Fluorescent Sensor for Cyanide Detection.

ABSTRACT: Peptoids, N-substituted glycine oligomers, are versatile peptidomimetics with diverse biomedical applications. However, strategies to the development of novel fluorescent peptoids as chemical sensors have not been extensively explored, yet. Here, we synthesized a novel peptoid-based fluorescent probe in which a coumarin moiety was incorporated via copper(I)-catalyzed azide-alkyne cycloaddition reaction. Fluorescence of the newly generated coumarin-peptoid was dramatically quenched upon coordination of the Cu(2+) ion, and the resulting peptoid-Cu(2+) complex exhibited significant Turn-ON fluorescence following the addition of CN(-). The rapid and reversible response, combined with cyanide selectivity of the synthesized peptoid, reflects a multistep photo-process and supports its utility as a new type of CN(-) sensor.

SUBMITTER: Lim B

PROVIDER: S-EPMC6273317 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

A Peptoid-Based Fluorescent Sensor for Cyanide Detection.

Lim Bumhee B Lee Jeeyeon J

Molecules (Basel, Switzerland) 20160310 3

Peptoids, N-substituted glycine oligomers, are versatile peptidomimetics with diverse biomedical applications. However, strategies to the development of novel fluorescent peptoids as chemical sensors have not been extensively explored, yet. Here, we synthesized a novel peptoid-based fluorescent probe in which a coumarin moiety was incorporated via copper(I)-catalyzed azide-alkyne cycloaddition reaction. Fluorescence of the newly generated coumarin-peptoid was dramatically quenched upon coordinat ...[more]

PMID: 26978334

Similar Datasets

Molecularly Imprinted Magnetic Fluorescent Nanocomposite-Based Sensor for Selective Detection of Lysozyme.

Project description:A new strategy for the design and construction of molecularly imprinted magnetic fluorescent nanocomposite-based-sensor is proposed. This multifunctional nanocomposite exhibits the necessary optics, magnetism and biocompatibility for use in the selective fluorescence detection of lysozyme. The magnetic fluorescent nanocomposites are prepared by combining carboxyl- functionalized Fe3O4 magnetic nanoparticles with l-cysteine-modified zinc sulfide quantum dots (MNP/QDs). Surface molecular imprinting technology was employed to coat the lysozyme molecularly imprinted polymer (MIP) layer on the MNP/QDs to form a core-shell structure. The molecularly imprinted MNP/QDs (MNP/QD@MIPs) can rapidly separate the target protein and then use fluorescence sensing to detect the protein; this reduces the background interference, and the selectivity and sensitivity of the detection are improved. The molecularly imprinted MNP/QDs sensor presented good linearity over a lysozyme concentration range from 0.2 to 2.0 μM and a detection limit of 4.53 × 10-3 μM for lysozyme. The imprinting factor of the MNP/QD@MIPs was 4.12, and the selectivity coefficient ranged from 3.19 to 3.85. Furthermore, the MNP/QD@MIPs sensor was applied to detect of lysozyme in human urine and egg white samples with recoveries of 95.40-103.33%. Experimental results showed that the prepared MNP/QD@MIPs has potential for selective magnetic separation and fluorescence sensing of target proteins in biological samples.

| S-EPMC8232576 | biostudies-literature

A modular platform to develop peptoid-based selective fluorescent metal sensors.

Project description:Despite the reduction in industrial use of toxic heavy metals, there remain contaminated natural water sources across the world. Herein we present a modular platform for developing selective sensors for toxic metal ions using N-substituted glycine, or peptoid, oligomers coupled to a fluorophore. As a preliminary evaluation of this strategy, structures based on previously identified metal-binding peptoids were synthesized with terminal pyrene moieties. Both derivatives of this initial design demonstrated a turn-off response in the presence of various metal ions. A colorimetric screen was designed to identify a peptoid ligand that chelates Hg(ii). Multiple ligands were identified that were able to deplete Hg(ii) from a solution selectively in the presence of an excess of competing ions. The C-terminal fluoropeptoid derivatives demonstrated similar selectivity to their label-free counterparts. This strategy could be applied to develop sensors for many different metal ions of interest using a variety of fluorophores, leading to a panel of sensors for identifying various water source contaminants.

| S-EPMC5697149 | biostudies-literature

"Off-on" aggregation-based fluorescent sensor for the detection of chloride in water.

Project description:Receptors selective for anions in aqueous media are a crucial component in the detection of anions for biological and environmental applications. Recent sensor designs have taken advantage of systems known to aggregate in solution, eliciting a fluorescent response. Herein, we demonstrate a chloride-selective fluorescent response of receptor 1(+), based on our well-established class of 2,6-bis(2-anilinoethynyl)pyridine bisureas. The fluorescence intensity ratio of 1(+)·Cl(->) aggregates in water is four times larger than the next most fluorescent anion complex, 1(+)·ClO4(->). In addition, (1)H NMR spectroscopic titrations demonstrate 1(+) binds chloride more strongly than other biologically relevant anions in solutions of both DMSO-d6 and 50/50 DMSO-d6/MeCN-d3.

| S-EPMC4537155 | biostudies-literature

Selectively Light-up Detection of Phosgene with an Aggregation-Induced Emission-Based Fluorescent Sensor.

Project description:Phosgene, a widely used but highly toxic substance, may pose a serious risk to public safety and health because of the potential abuse and possible accidental leakage. Consequently, it is of great significance to develop a rapid, reliable, and sensitive detection method for this noxious agent. In this work, an aggregation-induced emission-based sensor, 3,6-bis(1,2,2-triphenylvinyl)benzene-1,2-diamine (DATPE), has been rationally designed for detecting phosgene by conjugation of o-phenylenediamine (OPD) core as the reactive recognition moiety decorated with two peripheral triphenylethylene (TPE) units. A light-up fluorescence response is achieved by the fast cyclization reaction of OPD part and phosgene along with the formation of 2-imidazolidinone ring, thus inhibiting the intramolecular charge transfer quenching process in the sensor. Moreover, an easy-to-use test paper with DATPE is fabricated for onsite visual detection of phosgene in the gas phase even at a concentration of as low as 0.1 ppm.

| S-EPMC6941368 | biostudies-literature

A smart deoxyribozyme-based fluorescent sensor for in vitro detection of androgen receptor mRNA.

Project description:Nowadays a variety of biosensors are widely used in different fields, including biomedical diagnostics and self-testing. Nucleic acid-based biosensors are typically applied to detect another nucleic acid, proteins, ions, and several other types of compounds. It is most promising to develop simple and effective biosensors for the use in situations where traditional methods are not available due to their complexity and laboriousness. In this project, a novel smart deoxyribozyme-based fluorescent sensor for the detection of androgen receptor mRNA was developed. It consists of several functional modules including two deoxyribozymes 10-23, an RNA-dependent split malachite green aptamer, and an oligonucleotide platform. Deoxyribozymes specifically release a 27-nucleotide RNA fragment that is readily available for the interaction with the aptamer module. This solves a problem of secondary structure in hybridization with the target sequence of full-length mRNA. It was shown that within 24 hours the proposed sensor specifically recognized both a synthetic 60-nucleotide RNA fragment (LOD is 1.4 nM of RNA fragment at 37 °C) and a full-sized mRNA molecule of the androgen receptor. The constructed sensor is easy to use, has high efficiency and selectivity for the RNA target, and can be reconstructed for the detection of various nucleic acid sequences due to its modular structure. Thus, similar biosensors may be useful for the differential diagnosis.

| S-EPMC7277777 | biostudies-literature

An AuNPs-Based Fluorescent Sensor with Truncated Aptamer for Detection of Sulfaquinoxaline in Water.

Project description:Herein, we developed a novel truncation technique for aptamer sequences to fabricate highly sensitive aptasensors based on molecular docking and molecular dynamics simulations. The binding mechanism and energy composition of the aptamer/sulfaquinoxaline (SQX) complexes were investigated. We successfully obtained a new SQX-specific aptamer (SBA28-1: CCCTAGGGG) with high affinity (Kd = 27.36 nM) and high specificity determined using graphene oxide. This aptamer has a unique stem-loop structure that can bind to SQX. Then, we fabricated a fluorescence aptasensor based on SBA28-1, gold nanoparticles (AuNPs), and rhodamine B (RhoB) that presented a good linear range of 1.25-160 ng/mL and a limit of detection of 1.04 ng/mL. When used to analyze water samples, the aptasensor presented acceptable recovery rates of 93.1-100.1% and coefficients of variation (CVs) of 2.2-10.2%. In conclusion, the fluorescence aptasensor can accurately and sensitively detect SQX in water samples and has good application prospects.

| S-EPMC9312917 | biostudies-literature

Fluorescent Sensor for Copper(II) and Cyanide Ions via the Complexation-Decomplexation Mechanism with Di(bissulfonamido)spirobifluorene.

Project description:A novel spirobifluorene derivative bearing two bissulfonamido groups is successfully synthesized by Sonogashira coupling. This compound exhibits a strong fluorescence quenching by Cu(II) ion in a 50% mixture between acetonitrile and 20 mM pH 7.0 N-(2-hydroxyethyl)piperazine-N'-ethanesulfonic acid (HEPES) buffer with a detection limit of 98.2 nM. However, this sensor also shows ratiometric signal shifts from blue to yellow in the presence of Zn(II), Pb(II), and Hg(II) ions. The static quenching mechanism is verified by the signal reversibility using ethylenediaminetetraacetic acid (EDTA) and the Stern-Volmer plots at varying temperatures. The Cu(II)-spirobifluorene complex shows a highly selective fluorescence enhancement upon the addition of CN- ion with the detection limit of 390 nM. The application of this complex for quantitative analysis of spiked CN- ion in real water samples resulted in good recoveries.

| S-EPMC8246698 | biostudies-literature

An Nd3+-Sensitized Upconversion Fluorescent Sensor for Epirubicin Detection.

Project description:We describe here an Nd3+-sensitized upconversion fluorescent sensor for epirubicin (EPI) detection in aqueous solutions under 808 nm laser excitation. The upconversion fluorescence of nanoparticles is effectively quenched in the presence of EPI via a fluorescence resonance energy transfer mechanism. The dynamic quenching constant was 2.10 × 104 M-1. Normalized fluorescence intensity increased linearly as the EPI concentration was raised from 0.09 ?M to 189.66 ?M and the fluorometric detection limit was 0.05 ?M. The sensing method was simple, fast, and low-cost and was able to be applied to determine the levels of EPI in urine with spike recoveries from 97.5% to 102.6%. Another important feature of the proposed fluorescent sensor is that it holds a promising potential for in vivo imaging and detection due to its distinctive properties such as weak autofluorescence, low heating effect, and high light penetration depth.

| S-EPMC6955805 | biostudies-literature

Development of the PANI/MWCNT Nanocomposite-Based Fluorescent Sensor for Selective Detection of Aqueous Ammonia.

Project description:The present work reported the polyaniline (PANI) and multiwalled carbon nanotube (MWCNT)-based nanocomposite as a sensing material for the determination of aqueous ammonia by the enhanced fluorescence method. The excitation wavelength-dependent photoluminescence (PL) intensity has shown dual emission peaks at 340 and 380 nm that correspond to two different excitation energy states. The pH-based PL intensity and zeta potential variation were analyzed to optimize the suitable medium for aqueous ammonia sensing. Zeta potential was found to shift from 4 to -21 mV upon changing the pH of the the solution from acidic to alkaline medium. The fluorescence intensity of PANI/MWCNTs was found to increase upon increasing the pH from 3.0 to 6.0 (acidic region) and exhibits a plateau upon further increasing the pH from 7.0 to 12 (basic region). The PANI/MWCNT composite has shown a linear response to aqueous ammonia concentration varying from 25 to 200 ?M with a correlation coefficient (R 2) of 0.99 and a limit of detection of 15.19 ?M. The presence of relevant interference molecules and physiological ions had no influence on the detection of aqueous ammonia. Field-level study demonstrated that the level of aqueous ammonia can be determined selectively by using the PANI/MWCNT composite for various applications. The mechanism for the selective detection of aqueous ammonia is deliberated in detail.

| S-EPMC7178370 | biostudies-literature

Smart-phone, paper-based fluorescent sensor for ultra-low inorganic phosphate detection in environmental samples.

Project description:A major goal of environmental agencies today is to conduct point-of-collection monitoring of excess inorganic phosphate (Pi) in environmental water samples for tracking aquatic "dead zones" caused by algae blooms. However, there are no existing commercial devices which have been miniaturized and are suitable for the point-of-need-testing ("PONT") that is required to fully map a large region, such as the Florida Everglades. To solve this challenge, a reflection-mode fluorescence-sensing apparatus was developed, leveraging an environmentally sensitive fluorophore (MDCC) bound to a bacterial phosphate-binding protein to generate a fluorescent optical signal proportional to the concentration of (Pi) present. The combined end-to-end integrated sensor system had a response time of only 4 s, with minimal effects of common interfering agents and a linear range spanning from 1.1 to 64 ppb. To support ease-of-use during PONT, the platform incorporated disposable wax-printed paper strip sample pads and a smartphone camera detection system. Since the EPA threshold is currently 30 ppb to prevent eutrophication, this system serves as a rapid test of whether a region is compliant.

| S-EPMC6803704 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data