Calcineurin promotes APC/C activation at meiotic exit by acting on both XErp1 and Cdc20.

ABSTRACT: Vertebrate oocytes await fertilization arrested at metaphase of the second meiotic division. Fertilization triggers a transient calcium wave, which induces the activation of the anaphase-promoting complex/cyclosome (APC/C) and its co-activator Cdc20 resulting in the destruction of cyclin B and hence meiotic exit. Two calcium-dependent enzymes are implicated in fertilization-induced APC/C^Cdc20 activation: calcium-/calmodulin-dependent kinase type II (CaMKII) and calcineurin (CaN). While the role of CaMKII in targeting the APC/C inhibitor XErp1/Emi2 for destruction is well-established, it remained elusive how CaN affects APC/C^Cdc20 activation. Here, we discover that CaN contributes to APC/C^Cdc20 activation in Xenopus laevis

SUBMITTER: Heim A 

PROVIDER: S-EPMC6280790 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature