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A Photoenzymatic NADH Regeneration System.

ABSTRACT: A photoenzymatic NADH regeneration system was established. The combination of deazariboflavin as a photocatalyst with putidaredoxin reductase enabled the selective reduction of NAD+ into the enzyme-active 1,4-NADH to promote an alcohol dehydrogenase catalysed stereospecific reduction reaction. The catalytic turnover of all the reaction components was demonstrated. Factors influencing the efficiency of the overall system were identified.

SUBMITTER: Hofler GT

PROVIDER: S-EPMC6283237 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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A Photoenzymatic NADH Regeneration System.

Höfler Georg T GT Fernández-Fueyo Elena E Pesic Milja M Younes Sabry H SH Choi Eun-Gyu EG Kim Yong H YH Urlacher Vlada B VB Arends Isabel W C E IWCE Hollmann Frank F

Chembiochem : a European journal of chemical biology 20181023 22

A photoenzymatic NADH regeneration system was established. The combination of deazariboflavin as a photocatalyst with putidaredoxin reductase enabled the selective reduction of NAD<sup>+</sup> into the enzyme-active 1,4-NADH to promote an alcohol dehydrogenase catalysed stereospecific reduction reaction. The catalytic turnover of all the reaction components was demonstrated. Factors influencing the efficiency of the overall system were identified. ...[more]

PMID: 30192991

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