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Retinoic acid improves maturation rate and upregulates the expression of antioxidant-related genes in in vitro matured buffalo (Bubalus bubalis) oocytes.


ABSTRACT: Retinoic acid, vitamin A metabolite, plays a role in oocyte development and maturation in different ways including gene expression alteration and/or prohibiting oxidative stress. The objective of this study was to examine the effect of 9-cis-retinoic acid (9-cisRA) on the quality and maturation rate of buffalo oocytes. Cumulus-oocyte complexes (COCs, n?=?460) were collected from ovaries of slaughtered buffalos. Varying concentrations of 9-cisRA (0, 5, 50, and 200?nM) were added to the maturation medium, and the following parameters were analyzed: (i) maturation and cleavage rates, (ii) mitochondrial activity and reactive oxygen species (ROS) levels, (iii) expression level of antioxidant-related genes (PRDX1, SOD1, CAT, HOMX1, and GPX4) using RT-qPCR. Maturation rate was significantly improved in 5?nM 9-cisRA oocyte group (95.8%, P?

SUBMITTER: Gad A 

PROVIDER: S-EPMC6286416 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Retinoic acid improves maturation rate and upregulates the expression of antioxidant-related genes in <i>in vitro</i> matured buffalo (<i>Bubalus bubalis</i>) oocytes.

Gad Ahmed A   Abu Hamed Said S   Khalifa Mohamed M   Amin Ahmed A   El-Sayed Ashraf A   Swiefy Swiefy A SA   El-Assal Salah S  

International journal of veterinary science and medicine 20180915 2


Retinoic acid, vitamin A metabolite, plays a role in oocyte development and maturation in different ways including gene expression alteration and/or prohibiting oxidative stress. The objective of this study was to examine the effect of 9-cis-retinoic acid (9-cisRA) on the quality and maturation rate of buffalo oocytes. Cumulus-oocyte complexes (COCs, n = 460) were collected from ovaries of slaughtered buffalos. Varying concentrations of 9-cisRA (0, 5, 50, and 200 nM) were added to the maturation  ...[more]

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