CRISPR-Induced TMPRSS2-ERG Gene Fusions in Mouse Prostate Organoids.
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ABSTRACT:
SUBMITTER: Driehuis E
PROVIDER: S-EPMC6287496 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Publications
CRISPR-Induced TMPRSS2-ERG Gene Fusions in Mouse Prostate Organoids.
Driehuis Else E Clevers Hans H
JSM biotechnology & biomedical engineering 20170328 1
TMPRSS2-ERG fusions are common genetic events in prostate cancer. Until now, this genetic alteration was modelled by ERG overexpression. In this short communication, we report the creation of mouse prostate organoids that have undergone gene fusion through a CRISPR/Cas9-based strategy. The genetic fusion of TMPRSS2 and ERG results in ERG overexpression. This effect is androgen receptor-mediated, as expression of the fusion transcript can be restored to wildtype ERG levels by treatment with the a ...[more]
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