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Waste residues from Opuntia ficus indica for peroxidase-mediated preparation of phenolic dimeric compounds.

ABSTRACT: A methodology to detect peroxidase activity in Opuntia ficus indica cladodes waste extracts was performed and then used towards phenolic compounds. The extracts were able to dimerize three different molecules. Dimeric compounds were produced with yields ranging from 11% to 55%. The influence of H2O2 concentration was also tested, finding better yields when the peroxide-to-substrate ratio was 1:1. Some water-miscible solvents were used trying to increase overall yields, but no-significant positive results were found. In fact, one of them, THF, seemed to inhibit dimerization reaction. Hence, we have tested an alternative natural peroxidase source obtained from the wastes of a local highly-consumed vegetable and studied their enzymatic activity towards the preparation of biologically active, valuable compounds.

SUBMITTER: Sanchez-Carvajal AL

PROVIDER: S-EPMC6288046 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Waste residues from Opuntia ficus indica for peroxidase-mediated preparation of phenolic dimeric compounds.

Sánchez-Carvajal Ana Laura AL Alatorre-Santamaría Sergio S Valerio-Alfaro Gerardo G Hérnández-Vázquez Liliana L Navarro-Ocaña Arturo A

Biotechnology reports (Amsterdam, Netherlands) 20181103

A methodology to detect peroxidase activity in Opuntia ficus indica cladodes waste extracts was performed and then used towards phenolic compounds. The extracts were able to dimerize three different molecules. Dimeric compounds were produced with yields ranging from 11% to 55%. The influence of H2O2 concentration was also tested, finding better yields when the peroxide-to-substrate ratio was 1:1. Some water-miscible solvents were used trying to increase overall yield ...[more]

PMID: 30568885

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