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Transcriptome dataset of trunk neural crest cells migrating along the ventral pathway of chick embryos.


ABSTRACT: We present a transcriptome dataset generated from migratory chick trunk neural crest cells, which are destined to form components of the peripheral nervous system. Using the Sox10E1 enhancer, which specifically labels neural crest cells migrating on the trunk ventral pathway, we performed fluorescence activated cell sorting (FACS) of electroporated embryos to obtain a pure population of these cells for library preparation and Illumina sequencing. The results provide a list of genes that are enriched in the trunk neural crest. To validate the data, we performed in situ hybridization to visualize expression of selected transcripts.

SUBMITTER: Murko C 

PROVIDER: S-EPMC6288396 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Transcriptome dataset of trunk neural crest cells migrating along the ventral pathway of chick embryos.

Murko Christina C   Vieceli Felipe Monteleone FM   Bronner Marianne M  

Data in brief 20181127


We present a transcriptome dataset generated from migratory chick trunk neural crest cells, which are destined to form components of the peripheral nervous system. Using the Sox10E1 enhancer, which specifically labels neural crest cells migrating on the trunk ventral pathway, we performed fluorescence activated cell sorting (FACS) of electroporated embryos to obtain a pure population of these cells for library preparation and Illumina sequencing. The results provide a list of genes that are enri  ...[more]

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