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The study of multivalent carbohydrate-protein interactions by bio-layer interferometry.

ABSTRACT: The study of complex multivalent carbohydrate-protein interactions remains highly complicated and sometimes rendered impossible due to aggregation problems. In this study, we demonstrate that bio-layer interferometry is an excellent complementary method to standard techniques such as SPR and ITC. Using tetra- and hexadecavalent GalNAc glycoconjugates and Helix pomatia agglutinin (HPA) as a model lectin, we were able to measure reliable kinetic and thermodynamic parameters of multivalent interactions going from the micro to the nanomolar range.

SUBMITTER: Laigre E

PROVIDER: S-EPMC6289105 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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The study of multivalent carbohydrate-protein interactions by bio-layer interferometry.

Laigre Eugénie E Goyard David D Tiertant Claire C Dejeu Jérôme J Renaudet Olivier O

Organic & biomolecular chemistry 20181101 46

The study of complex multivalent carbohydrate-protein interactions remains highly complicated and sometimes rendered impossible due to aggregation problems. In this study, we demonstrate that bio-layer interferometry is an excellent complementary method to standard techniques such as SPR and ITC. Using tetra- and hexadecavalent GalNAc glycoconjugates and Helix pomatia agglutinin (HPA) as a model lectin, we were able to measure reliable kinetic and thermodynamic parameters of multivalent interact ...[more]

PMID: 30264842

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