Apolipoprotein E particle size is increased in Alzheimer's disease.

ABSTRACT: Introduction:Apolipoprotein E4 (apoE4) is the predominant risk factor for late-onset Alzheimer's disease (AD), but the question of which structural differences might explain its effect remains unclear. Methods:We compared high-density lipoprotein-like apoE particles from 12 AD and 10 control patients using size-exclusion chromatography. Results:ApoE particles from patients genotyped as ?4/?4 were 2.2 ± 0.3 times as massive as particles from ?3/?3 control subjects and 1.4 ± 0.1 times as massive as particles from ?3/?3 AD patients. The increased particle size was not because of incorporation of amyloid ? or apoE proteolysis products. Particles from AD patients genotyped as ?3/?3 were 1.59 ± 0.27 times as massive as ?3/?3 control subjects. Discussion:Increased particle size in AD is affected by A PO E genotype and by disease-related differences in assembly or stability. These differences suggest that lipoprotein assembly or stability in AD brain plays an important role in determining apoE4 pathogenicity.

SUBMITTER: Nelson TJ 

PROVIDER: S-EPMC6293020 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature