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MYC Induces a Hybrid Energetics Program Early in Cell Reprogramming.

ABSTRACT: Cell reprogramming is thought to be associated with a full metabolic switch from an oxidative- to a glycolytic-based metabolism. However, neither the dynamics nor the factors controlling this metabolic switch are fully understood. By using cellular, biochemical, protein array, metabolomic, and respirometry analyses, we found that c-MYC establishes a robust bivalent energetics program early in cell reprogramming. Cells prone to undergo reprogramming exhibit high mitochondrial membrane potential and display a hybrid metabolism. We conclude that MYC proteins orchestrate a rewiring of somatic cell metabolism early in cell reprogramming, whereby somatic cells acquire the phenotypic plasticity necessary for their transition to pluripotency in response to either intrinsic or external cues.

SUBMITTER: Prieto J

PROVIDER: S-EPMC6294174 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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MYC Induces a Hybrid Energetics Program Early in Cell Reprogramming.

Prieto Javier J Seo Arnold Y AY León Marian M Santacatterina Fulvio F Torresano Laura L Palomino-Schätzlein Martina M Giménez Karen K Vallet-Sánchez Azahara A Ponsoda Xavier X Pineda-Lucena Antonio A Cuezva José M JM Lippincott-Schwartz Jennifer J Torres Josema J

Stem cell reports 20181121 6

Cell reprogramming is thought to be associated with a full metabolic switch from an oxidative- to a glycolytic-based metabolism. However, neither the dynamics nor the factors controlling this metabolic switch are fully understood. By using cellular, biochemical, protein array, metabolomic, and respirometry analyses, we found that c-MYC establishes a robust bivalent energetics program early in cell reprogramming. Cells prone to undergo reprogramming exhibit high mitochondrial membrane potential a ...[more]

PMID: 30472011

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