Ontology highlight
ABSTRACT:
SUBMITTER: Kim JS
PROVIDER: S-EPMC6294903 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
Kim Ju-Sim JS Liu Lin L Fitzsimmons Liam F LF Wang Yang Y Crawford Matthew A MA Mastrogiovanni Mauricio M Trujillo Madia M Till James Karl A JKA Radi Rafael R Dai Shaodong S Vázquez-Torres Andrés A
Proceedings of the National Academy of Sciences of the United States of America 20181114 50
RNA polymerase is the only known protein partner of the transcriptional regulator DksA. Herein, we demonstrate that the chaperone DnaJ establishes direct, redox-based interactions with oxidized DksA. Cysteine residues in the zinc finger of DksA become oxidized in <i>Salmonella</i> exposed to low concentrations of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). The resulting disulfide bonds unfold the globular domain of DksA, signaling high-affinity interaction of the C-terminal α-helix to DnaJ. ...[more]