Genistein, daidzein, and resveratrols stimulate PGC-1?-mediated gene expression.

ABSTRACT: PGC-1? is a transcriptional co-activator of nuclear receptors such as the estrogen receptor-related receptor (ERR). Transgenic overexpression of PGC-1? in mice increases energy expenditure and suppresses high-fat diet-induced obesity. In this study, we screened various food-derived and natural compounds using a reporter assay system to measure the transcriptional activity of PGC-1?. Soy-derived isoflavones, genistein and daidzein, and several resveratrols activated PGC-1?. Genistein, daidzein, and trans-oxyresveratrol activated ERR-responsive element-mediated reporter activity in the presence of PGC-1?. Stable overexpression of PGC-1? in C2C12 myoblasts increased the expression of medium-chain acyl-CoA dehydrogenase (MCAD), an important enzyme in fatty acid ?-oxidation. Genistein and daidzein increased MCAD mRNA levels and mitochondrial content in PGC-1?-expressing C2C12 cells. These compounds activated ERR/PGC-1? complex-mediated gene expression, and our findings may be a practical foundation for developing functional foods targeting obesity.

SUBMITTER: Uchitomi R 

PROVIDER: S-EPMC6295599 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature