Partial purification and characterization of serine protease produced through fermentation of organic municipal solid wastes by Serratia marcescens A3 and Pseudomonas putida A2.

ABSTRACT: Proteolytic bacteria isolated from municipal solid wastes (MSW) were identified as Serratia marcescens A3 and Pseudomonas putida A2 based on 16S rDNA sequencing. Protease produced through fermentation of organic MSW by these bacteria under some optimized physicochemical parameters was partially purified and characterized. The estimated molecular mass of the partially purified protease from S. marcescens and P. putida was approximately 25 and 38 kDa, respectively. Protease from both sources showed low K_m 0.3 and 0.5 mg ml^-1 and high V_max 333 and 500 µmole min^-1 at 40 °C, and thermodynamics analysis suggested formation of ordered enzyme-substrate (E-S) complexes. The activation energy (E_a) and temperature quot

SUBMITTER: Iqbal A 

PROVIDER: S-EPMC6296650 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature