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Paper-based Transwell assays: an inexpensive alternative to study cellular invasion.

ABSTRACT: Cellular movement is essential in the formation and maintenance of healthy tissues as well as in disease progression such as tumor metastasis. In this work, we describe a paper-based Transwell assay capable of quantifying cellular invasion through an extracellular matrix. The paper-based Transwell assays generate similar datasets, with equivalent reproducibility, to commercially available Transwell assays. With different culture configurations, we quantify invasion: upon addition of an exogenous factor or in the presence of medium obtained from other cell types, in an indirect or direct co-culture format whose medium composition is dynamically changing, and in a single-zone or parallel (96-zone) format.

SUBMITTER: Kenney RM

PROVIDER: S-EPMC6296866 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Paper-based Transwell assays: an inexpensive alternative to study cellular invasion.

Kenney Rachael M RM Loeser Adam A Whitman Nathan A NA Lockett Matthew R MR

The Analyst 20181201 1

Cellular movement is essential in the formation and maintenance of healthy tissues as well as in disease progression such as tumor metastasis. In this work, we describe a paper-based Transwell assay capable of quantifying cellular invasion through an extracellular matrix. The paper-based Transwell assays generate similar datasets, with equivalent reproducibility, to commercially available Transwell assays. With different culture configurations, we quantify invasion: upon addition of an exogenous ...[more]

PMID: 30328422

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