Dataset Information

Expression profile of long non?coding RNAs in cardiomyocytes exposed to acute ischemic hypoxia.

ABSTRACT: Acute myocardial infarction (AMI) is a life?threatening disease and seriously influences patient quality of life. Long non?coding RNAs (lncRNAs), an emerging class of non?coding genes, have attracted attention in research, however, whether lncRNAs serve a function in acute ischemic hypoxia remains to be elucidated. In the present study, an lncRNA microarray was used to analyze differential lncRNA expression in acute ischemic hypoxia. A total of 323 lncRNAs were identified, 168 of which were upregulated and 155 of which were downregulated. Gene Ontology and Pathway analyses were also used to identify the potential functions of dysregulated lncRNAs; it was predicted that these dysregulated lncRNAs may contribute to the initiation of AMI. It was demonstrated that an lncRNA termed sloyfley may influence acute ischemic hypoxia through its neighboring gene Peg3, which has been linked to brain ischemia hypoxia. In summary, the present study identified numerous lncRNAs, which may provide further opportunities for the development of novel therapeutic strategies.

SUBMITTER: Li H

PROVIDER: S-EPMC6297740 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Expression profile of long non‑coding RNAs in cardiomyocytes exposed to acute ischemic hypoxia.

Li Hua H Cheng Zijie Z Tang Yuanyuan Y Feng Mengwen M Yin Anwen A Zhang Hao H Xu Jia J Zhang Qijun Q Zhang Jinsong J Qian Lingmei L

Molecular medicine reports 20181113 1

Acute myocardial infarction (AMI) is a life‑threatening disease and seriously influences patient quality of life. Long non‑coding RNAs (lncRNAs), an emerging class of non‑coding genes, have attracted attention in research, however, whether lncRNAs serve a function in acute ischemic hypoxia remains to be elucidated. In the present study, an lncRNA microarray was used to analyze differential lncRNA expression in acute ischemic hypoxia. A total of 323 lncRNAs were identified, 168 of which were upre ...[more]

PMID: 30431112

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Project description:Long non?coding RNAs (lncRNAs) represent potential biomarkers for the diagnosis and treatment of various diseases; however, the role of circulating acute ischemic stroke (AIS)?related lncRNAs remains relatively unknown. The present study aimed to screen crucial lncRNAs for AIS based on the competing endogenous RNA (ceRNA) hypothesis. The expression profile datasets for one mRNA, accession no. GSE16561, and four microRNAs (miRNAs), accession nos. GSE95204, GSE86291, GSE55937 and GSE110993, were downloaded from the Gene Expression Omnibus database. Differentially expressed genes (DEGs), lncRNAs (DELs), and miRNAs (DEMs) were identified, and ClusterProfiler was used to interpret the function of the DEGs. Based on the protein?protein interaction (PPI) network and module analyses, hub DEGs were identified. A ceRNA network was established based on miRNA?mRNA or miRNA?lncRNA interaction pairs. In total, 2,041 DEGs and 5 DELs were identified between the AIS and controls samples in GSE16561, and 10 DEMs between at least two of the four miRNA expression profiles. A PPI network was constructed with 1,235 DEGs, among which 20 genes were suggested to be hub genes. The hub genes paxillin (PXN), FYN?proto?oncogene, Src family tyrosine kinase (FYN), ras homolog family member A (RHOA), STAT1, and growth factor receptor?bound protein 2 (GRB2), were amongst the most significantly enriched modules extracted from the PPI network. Functional analysis revealed that these hub genes were associated with inflammation?related signaling pathways. An AIS?related ceRNA network was constructed, in which 4 DELs were predicted to function as ceRNAs for 9 DEMs, to regulate the five identified hub genes; that is, minichromosome maintenance complex component 3 associated protein?antisense RNA 1 (MCM3AP?AS1)/long intergenic non?protein coding RNA 1089 (LINC01089)/hsa?miRNA (miR)?125a/FYN, inositol?tetrakisphosphate 1?kinase?antisense RNA 1 (ITPK1?AS1)/hsa?let?7i/RHOA/GRB2/STAT1, and human leukocyte antigen complex group 27 (HCG27)/hsa?-miR?19a/PXN interaction axes. In conclusion, MCM3AP?AS1, LINC01089, ITPK1?AS1, and HCG27 may represent new biomarkers and underlying targets for the treatment of AIS.

Dataset Information

Expression profile of long non?coding RNAs in cardiomyocytes exposed to acute ischemic hypoxia.

Publications

Expression profile of long non‑coding RNAs in cardiomyocytes exposed to acute ischemic hypoxia.

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