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Expression, purification, crystallization and preliminary X-ray diffraction analysis of swine leukocyte antigen 2 complexed with a CTL epitope AS64 derived from Asia1 serotype of foot-and-mouth disease virus.


ABSTRACT: BACKGROUND:Currently, the structural characteristics of the swine major histocompatibility complex (MHC) class I molecule, also named swine leukocyte antigen class I (SLA-I) molecule need to be further clarified. RESULTS:A complex of SLA-I constituted by an SLA-2*HB01 molecule with swine ?2-microglobulin and a cytotoxic T lymphocyte (CTL) epitope FMDV-AS64 (ALLRSATYY) derived from VP1 protein (residues 64-72) of Asia 1 serotype of foot-and-mouth disease virus (FMDV) was expressed, refolded, purified and crystallized. By preliminary X-ray diffraction analysis, it was shown that the diffraction resolution of the crystal was 2.4?Å and the space group belonged to P212121 with unit cell parameters a?=?48.37, b?=?97.75, c?=?166.163?Å. CONCLUSION:This research will be in favor of illuminating the structural characteristics of an SLA-2 molecule associated with a CTL epitope derived from Asia1 serotype of FMDV.

SUBMITTER: Gao FS 

PROVIDER: S-EPMC6299498 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Expression, purification, crystallization and preliminary X-ray diffraction analysis of swine leukocyte antigen 2 complexed with a CTL epitope AS64 derived from Asia1 serotype of foot-and-mouth disease virus.

Gao Feng-Shan FS   Feng Lei L   Jiang Ping P   Li Zi-Bin ZB   Gao Hua H   Zhai Xiao-Xin XX   Zhang Zong-Hui ZH   Hu Xiao X  

BMC veterinary research 20181218 1


<h4>Background</h4>Currently, the structural characteristics of the swine major histocompatibility complex (MHC) class I molecule, also named swine leukocyte antigen class I (SLA-I) molecule need to be further clarified.<h4>Results</h4>A complex of SLA-I constituted by an SLA-2*HB01 molecule with swine β<sub>2</sub>-microglobulin and a cytotoxic T lymphocyte (CTL) epitope FMDV-AS64 (ALLRSATYY) derived from VP1 protein (residues 64-72) of Asia 1 serotype of foot-and-mouth disease virus (FMDV) was  ...[more]

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