Dataset Information

Imaging glial activation in patients with post-treatment Lyme disease symptoms: a pilot study using [11C]DPA-713 PET.

ABSTRACT: The pathophysiology of post-treatment Lyme disease syndrome (PTLDS) may be linked to overactive immunity including aberrant activity of the brain's resident immune cells, microglia. Here we used [11C]DPA-713 and positron emission tomography to quantify the 18?kDa translocator protein, a marker of activated microglia or reactive astrocytes, in the brains of patients with post-treatment Lyme disease symptoms of any duration compared to healthy controls. Genotyping for the TSPO rs6971 polymorphism was completed, and individuals with the rare, low affinity binding genotype were excluded. Data from eight brain regions demonstrated higher [11C]DPA-713 binding in 12 patients relative to 19 controls. [11C]DPA-713 PET is a promising tool to study cerebral glial activation in PTLDS and its link to cognitive symptoms.

SUBMITTER: Coughlin JM

PROVIDER: S-EPMC6299943 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Imaging glial activation in patients with post-treatment Lyme disease symptoms: a pilot study using [<sup>11</sup>C]DPA-713 PET.

Coughlin Jennifer M JM Yang Ting T Rebman Alison W AW Bechtold Kathleen T KT Du Yong Y Mathews William B WB Lesniak Wojciech G WG Mihm Erica A EA Frey Sarah M SM Marshall Erica S ES Rosenthal Hailey B HB Reekie Tristan A TA Kassiou Michael M Dannals Robert F RF Soloski Mark J MJ Aucott John N JN Pomper Martin G MG

Journal of neuroinflammation 20181219 1

The pathophysiology of post-treatment Lyme disease syndrome (PTLDS) may be linked to overactive immunity including aberrant activity of the brain's resident immune cells, microglia. Here we used [<sup>11</sup>C]DPA-713 and positron emission tomography to quantify the 18 kDa translocator protein, a marker of activated microglia or reactive astrocytes, in the brains of patients with post-treatment Lyme disease symptoms of any duration compared to healthy controls. Genotyping for the TSPO rs6971 po ...[more]

PMID: 30567544

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Project description:BACKGROUND:Cerebral stroke is a severe and frequent condition that requires rapid and reliable diagnosis. If administered shortly after the first symptoms manifest themselves, IV thrombolysis has been shown to increase the functional prognosis by restoring brain reperfusion. However, a better understanding of the pathophysiology of stroke should help to identify potential new therapeutic targets. Stroke is known to induce an inflammatory brain reaction that involves overexpression of the 18-kDa translocator protein (TSPO) in glial cells and infiltrated leukocytes, which can be visualised by positron emission tomography (PET). We aimed to evaluate post-stroke neuroinflammation using the PET TSPO radioligand (18)?F-DPA-714. METHODS:Nine patients underwent (18)?F-DPA-714 PET and magnetic resonance imaging (MRI) between 8 and 18 days after the ictus. Co-registration of MRI and PET images was used to define three volumes of interest (VOIs): core infarction, contralateral region, and cerebellum ipsilateral to the stroke lesion. Time activity curves were obtained from each VOI, and ratios of mean and maximum activities between the VOIs were calculated. RESULTS:We observed an increased uptake of (18)?F-DPA-714 co-localised with the infarct tissue and extension beyond the region corresponding to the damage in the blood brain barrier. No correlation was identified between (18)?F-DPA-714 uptake and infarct volume. (18)?F-DPA-714 uptake in ischemic lesion (mainly associated with TSPO expression in the infarct area and in the surrounding neighbourhood) slowly decreased from 10 min pi to the end of the PET acquisition, remaining higher than that in both contralateral region and ipsilateral cerebellum. CONCLUSION:Our results show that (18)?F-DPA-714 uptake after acute ischemia is mainly associated with TSPO expression in the infarct area and in the surrounding neighbourhood. We also demonstrated that the kinetics of (18)?F-DPA-714 differs in injured tissue compared to normal tissue. Therefore, (18)?F-DPA-714 may be useful in assessing the extent of neuroinflammation associated with acute stroke and could also help to predict clinical outcomes and functional recovery, as well as to assess therapeutic strategies, such as the use of neuroprotective/anti-inflammatory drugs.

Dataset Information

Imaging glial activation in patients with post-treatment Lyme disease symptoms: a pilot study using [11C]DPA-713 PET.

Publications

Imaging glial activation in patients with post-treatment Lyme disease symptoms: a pilot study using [<sup>11</sup>C]DPA-713 PET.

Similar Datasets

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