Ontology highlight
ABSTRACT:
SUBMITTER: Brynnel A
PROVIDER: S-EPMC6300359 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
Brynnel Ambjorn A Hernandez Yaeren Y Kiss Balazs B Lindqvist Johan J Adler Maya M Kolb Justin J van der Pijl Robbert R Gohlke Jochen J Strom Joshua J Smith John J Ottenheijm Coen C Granzier Henk L HL
eLife 20181219
Titin, the largest protein known, forms an elastic myofilament in the striated muscle sarcomere. To establish titin's contribution to skeletal muscle passive stiffness, relative to that of the extracellular matrix, a mouse model was created in which titin's molecular spring region was shortened by deleting 47 exons, the <i>Ttn<sup>Δ112-158</sup></i> model. RNA sequencing and super-resolution microscopy predicts a much stiffer titin molecule. Mechanical studies with this novel mouse model support ...[more]