Functional Status of Neuronal Calcium Sensor-1 Is Modulated by Zinc Binding.

ABSTRACT: Neuronal calcium sensor-1 (NCS-1) protein is abundantly expressed in the central nervous system and retinal neurons, where it regulates many vital processes such as synaptic transmission. It coordinates three calcium ions by EF-hands 2-4, thereby transducing Ca²⁺ signals to a wide range of protein targets, including G protein-coupled receptors and their kinases. Here, we demonstrate that NCS-1 also has Zn²⁺-binding sites, which affect its structural and functional properties upon filling. Fluorescence and circular dichroism experiments reveal the impact of Zn²⁺ binding on NCS-1 secondary and tertiary structure. According to atomic absorption spectroscopy and isothermal titration calorimetry studies, apo-NCS-1 has two high-affinity (4 × 10⁶ M

SUBMITTER: Tsvetkov PO 

PROVIDER: S-EPMC6302015 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature