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Corynebacterium Cell Factory Design and Culture Process Optimization for Muconic Acid Biosynthesis.


ABSTRACT: Muconic acid (MA) is a valuable compound for adipic acid production, which is a precursor for the synthesis of various polymers such as plastics, coatings, and nylons. Although MA biosynthesis has been previously reported in several bacteria, the engineered strains were not satisfactory owing to low MA titers. Here, we generated an engineered Corynebacterium cell factory to produce a high titer of MA through 3-dehydroshikimate (DHS) conversion to MA, with heterologous expression of foreign protocatechuate (PCA) decarboxylase genes. To accumulate key intermediates in the MA biosynthetic pathway, aroE (shikimate dehydrogenase gene), pcaG/H (PCA dioxygenase alpha/beta subunit genes) and catB (chloromuconate cycloisomerase gene) were disrupted. To accomplish the conversion of PCA to catechol (CA), a step that is absent in Corynebacterium, a codon-optimized heterologous PCA decarboxylase gene was expressed as a single operon under the strong promoter in a aroE-pcaG/H-catB triple knock-out Corynebacterium strain. This redesigned Corynebacterium, grown in an optimized medium, produced about 38?g/L MA and 54?g/L MA in 7-L and 50-L fed-batch fermentations, respectively. These results show highest levels of MA production demonstrated in Corynebacterium, suggesting that the rational cell factory design of MA biosynthesis could be an alternative way to complement petrochemical-based chemical processes.

SUBMITTER: Lee HN 

PROVIDER: S-EPMC6303301 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Corynebacterium Cell Factory Design and Culture Process Optimization for Muconic Acid Biosynthesis.

Lee Han-Na HN   Shin Woo-Shik WS   Seo Seung-Yeul SY   Choi Si-Sun SS   Song Ji-Soo JS   Kim Ji-Yeon JY   Park Ji-Hoon JH   Lee Dohoon D   Kim Sang Yong SY   Lee Sang Joung SJ   Chun Gie-Taek GT   Kim Eung-Soo ES  

Scientific reports 20181221 1


Muconic acid (MA) is a valuable compound for adipic acid production, which is a precursor for the synthesis of various polymers such as plastics, coatings, and nylons. Although MA biosynthesis has been previously reported in several bacteria, the engineered strains were not satisfactory owing to low MA titers. Here, we generated an engineered Corynebacterium cell factory to produce a high titer of MA through 3-dehydroshikimate (DHS) conversion to MA, with heterologous expression of foreign proto  ...[more]

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