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Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification.

ABSTRACT: Apple stem grooving virus (ASGV) is considered to cause the most economically important viral disease in pears in Korea. The current PCR-based methods used to diagnose ASGV are time-consuming in terms of target detection. In this study, a novel assay for specific ASGV detection that is based on reverse transcription-recombinase polymerase amplification is described. This assay has been shown to be reproducible and able to detect as little as 4.7 ng/?l of purified RNA obtained from an ASGV-infected plant. The major advantage of this assay is that the reaction for the target virus is completed in 1 min, and amplification only requires an incubation temperature of 42°C. This assay is a promising alternative method for pear breeding programs or virus-free certification laboratories.

SUBMITTER: Kim NY

PROVIDER: S-EPMC6305176 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Rapid and Specific Detection of <i>Apple stem grooving virus</i> by Reverse Transcription-recombinase Polymerase Amplification.

Kim Nam-Yeon NY Oh Jonghee J Lee Su-Heon SH Kim Hongsup H Moon Jae Sun JS Jeong Rae-Dong RD

The plant pathology journal 20181201 6

<i>Apple stem grooving virus</i> (ASGV) is considered to cause the most economically important viral disease in pears in Korea. The current PCR-based methods used to diagnose ASGV are time-consuming in terms of target detection. In this study, a novel assay for specific ASGV detection that is based on reverse transcription-recombinase polymerase amplification is described. This assay has been shown to be reproducible and able to detect as little as 4.7 ng/μl of purified RNA obtained from an ASGV ...[more]

PMID: 30588230

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