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Expression and Characteristics of Two Glucose-Tolerant GH1 ?-glucosidases From Actinomadura amylolytica YIM 77502T for Promoting Cellulose Degradation.


ABSTRACT: The bioconversion of lignocellulose in various industrial processes, such as biofuel production, requires the degradation of cellulose. Actinomadura amylolytica YIM 77502T is an aerobic, Gram-positive actinomycete that can efficiently degrade crystalline cellulose by extracellular cellulases. Genomic analysis of A. amylolytica identified 9 cellulase and 11 ?-glucosidase genes that could potentially encode proteins that digest cellulose. Extracellular proteome characterization of A. amylolytica cell-free culture supernatant by liquid chromatography tandem mass spectrometry analysis revealed that 4 of these cellulases and 2 of these ?-glucosidases functioned during cellulose hydrolysis. Thin-layer chromatography analysis revealed extracellular ?-glucosidases play a major role in carboxyl methyl cellulose (CMC) degradation of products in culture supernatants. In this study, 2 of the identified secreted ?-glucosidases, AaBGL1 and AaBGL2, were functionally expressed in Escherichia coli and found to have ?-glucosidase activity with wide substrate specificities, including for p-nitrophenyl ?-D-glucopyranoside (pNPG), p-nitrophenyl-beta-D-cellobioside (pNPC), and cellobiose. Moreover, AaBGL1 and AaBGL2 had high tolerances for glucose. After adding these ?-glucosidases to commercial cellulases, the degradation rates of CMC, Avicel, birch sawdust, and corncob powder increased by 37, 42, 33, and 9%, respectively. Overall, this work identifies an alternative potential source of ?-glucosidases with potential applications in commercial cellulose utilization and the bioenergy industry.

SUBMITTER: Yin YR 

PROVIDER: S-EPMC6305311 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Expression and Characteristics of Two Glucose-Tolerant GH1 β-glucosidases From <i>Actinomadura amylolytica</i> YIM 77502<sup>T</sup> for Promoting Cellulose Degradation.

Yin Yi-Rui YR   Sang Peng P   Xian Wen-Dong WD   Li Xin X   Jiao Jian-Yu JY   Liu Lan L   Hozzein Wael N WN   Xiao Min M   Li Wen-Jun WJ  

Frontiers in microbiology 20181218


The bioconversion of lignocellulose in various industrial processes, such as biofuel production, requires the degradation of cellulose. <i>Actinomadura amylolytica</i> YIM 77502<sup>T</sup> is an aerobic, Gram-positive actinomycete that can efficiently degrade crystalline cellulose by extracellular cellulases. Genomic analysis of <i>A. amylolytica</i> identified 9 cellulase and 11 β-glucosidase genes that could potentially encode proteins that digest cellulose. Extracellular proteome characteriz  ...[more]

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