Ontology highlight
ABSTRACT:
SUBMITTER: Yelleswarapu M
PROVIDER: S-EPMC6305555 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
Yelleswarapu Maaruthy M van der Linden Ardjan J AJ van Sluijs Bob B Pieters Pascal A PA Dubuc Emilien E de Greef Tom F A TFA Huck Wilhelm T S WTS
ACS synthetic biology 20181119 12
Cell-free transcription-translation provides a simplified prototyping environment to rapidly design and study synthetic networks. Despite the presence of a well characterized toolbox of genetic elements, examples of genetic networks that exhibit complex temporal behavior are scarce. Here, we present a genetic oscillator implemented in an E. coli-based cell-free system under steady-state conditions using microfluidic flow reactors. The oscillator has an activator-repressor motif that utilizes the ...[more]