Ontology highlight
ABSTRACT:
SUBMITTER: Rossi NA
PROVIDER: S-EPMC6307708 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
Rossi Nicholas A NA Mora Thierry T Walczak Aleksandra M AM Dunlop Mary J MJ
PLoS computational biology 20181227 12
Several key transcription factors have unusually short half-lives compared to other cellular proteins. Here, we explore the utility of active degradation in shaping how the multiple antibiotic resistance activator MarA coordinates its downstream targets. MarA controls a variety of stress response genes in Escherichia coli. We modify its half-life either by knocking down the protease that targets it via CRISPRi or by engineering MarA to protect it from degradation. Our experimental and analytical ...[more]